Rajesh and latha, 2004 showed that various herbal extracts could protect organs. Ccl 4 is an hepatotoxic halomethane capable of causing hepatocellular fatty. The possible protective effect of kolaviron on rat erythrocytes followingsimultaneous administration of kolaviron 100 mgkg of body weightday withcarbon tetrachloride ccl4 1. Lipid peroxides are unstable and decompose to form a complex series of compounds including reactive carbonyl. Rosinduced lipid peroxidation has been implicated in neoplastic transformation 35. Carbon tetrachloridemediated lipid peroxidation induces. In conclusion, the results of this study demonstrate that bele can effectively prevent the ccl4induced hepatic damage in mice and, therefore, it can be used as a hepatoprotective agent. Such mitochondrial damage is not caused by ccl4 induced stimulation of lipid peroxidation since. Ccl4induced liver toxicity might be related to oxidative damage and lipid peroxidation. The aim of this study was to investigate whether pomegranate juice pj consumption has an ameliorating effect on carbon tetrachloride ccl 4induced sperm damages and testicular apoptosis associated with the oxidative stress in male.
Our aim was to investigate the bioavailability of t. Journal of medicinal plants research protective effects. Hepatoprotective effects of blue honeysuckle on ccl4. Evidence that silymarin acts both as an inhibitor of metabolic activation and as a chainbreaking antioxidant. Pdf effects of volatile oil constituents of nigella. Parboiled germinated brown rice protects against ccl4. Furthermore, pgbr inhibited degradation of cyp2e1 in ccl 4induced decrease of cyp2e1 activity. Mechanism of carbon tetrachlorideinduced hepatotoxicity. Since oxidative stress is implicated in the pathogenesis of liver diseases, this study was carried out to evaluate the hepatoprotective, hematoprotective and hypolipidemic effects of pleurotus ostreatus oyster mushroom in carbon tetrachloride ccl4 induced liver injury in wistar rats. Antioxidant and hepatoprotective potential of aegle. Res from dietary sources and its effect on an animal model of carbon tetrachloride ccl 4. Hepatoprotective effects of apple polyphenols on ccl4.
Ccl4 was induced in 42 male rats for eight weeks and checked the protective efficacy of methanolic extract of carissa opaca leaves at various hormonal imbalances, alteration of antioxidant enzymes, dna fragmentation levels and lipid peroxidation caused testicular fibrosis in testis while high performance liquid chromatography hplc was used. Ameliorating effect of pomegranate juice consumption on. In ccl 4 induced liver injury model, oxidative stress can provoke and promote lipid peroxidation that damage the hepatocellular. We hypothesized that turmeric extract and curcumin protect the liver from ccl4induced liver injury by reducing oxidative stress, inhibiting lipid peroxidation, and increasing glutathione peroxidase activation. Rats in two atmospheres of 100% o2 produced much less chcl3 and ethane than rats in air. Ccl4 was induced in 42 male rats for eight weeks and checked the protective efficacy of methanolic extract of carissa opaca leaves at various hormonal imbalances, alteration of antioxidant enzymes, dna fragmentation levels and lipid peroxidation caused testicular fibrosis in testis while high performance liquid chromatography hplc. While hypocalcemia was observed, there was no significant change in body weight. Mechanism for the protective effects of silymarin against. Effects of infliximab against carbon tetrachlorideinduced. Protective efficacy of natansnin, a dibenzoyl glycoside. Pdf antioxidant capacity of moringa oleifera seed oil. Production of chcl3 and ethane, the latter an index of lipid peroxidation, were measured. Production of reactive oxygen species and lipid peroxidation induced by iron overload bacon et al. Antioxidant and hepatoprotective effects of solanum.
In states of oxidative stress, gsh is converted to glutathione disulfide gssg leading to lipid peroxidation. On the other hand, high partial pressure of oxygen shifts ccl 4 metabolisms towards the formation of ccl 3oo radical with consequent lipid peroxidation and lead the cells from steatosis into apoptosis 39,41. Ast but not alt elevation induced by ccl4 was significantly alleviated in cll and curcumintreated rats. Lipid peroxidation was measured as mda in the experimental hamsters. Protection against carbon tetrachlorideinduced lipid peroxidation in the rat by dietary vitamin e, selenium and methionine as measured by ethane evolution. The antiperoxidative effects of 35 phenolic compounds, most of them belonging to the flavonoid class, were investigated using ccl4induced peroxidation of rat liver microsomes. Effects of thymoquinone on the changes of hepatic lipid peroxidation and total sulfhydryl content induced by ccl4 table 2 shows the effect of tq 12. This system was rather insensitive to gallic acid, methyl gallate and ellagic acid. Oxidative stress, lipid peroxidation, changes in the activity and content of metabolic enzymes, cytokines, apoptosis, and many other factors are involved in this process 11. The increment in lipid peroxidation, as assessed by the elevated levels of tbars following ccl 4 administration, has been well documented.
Changes in the levels of lipid peroxidation in ccl4 induced rats treated with aqueous extract of s. Silibinin, one of the three isomers composing silymarin, also decreased carbon tetrachlorideinduced lipid peroxidation. Nigella sativa seeds protect against hepatotoxicity and. However, lipid peroxidationindependent mechanisms seem to be involved in ccl 4 induced early hepatocyte swelling and changes in expression of stressdefenserelated genes. Inhalation of its vapors can depress central nervous system activity and cause degeneration of the liver and kidneys. Carbon tetrachloride ccl4 is used as a solvent for oils and fats, as a refrigerant, and as a drycleaning agent. Chronic hepatic stress was induced via intraperitoneal ip administration of a mixture of ccl4 0. Protective effects of carissa opaca fruits against ccl4. The protective effect of n acetyl cysteine against carbon. Protective effects of carissa opaca fruits against ccl4induced oxidative kidney lipid peroxidation and trauma in rat. Inhibitory effects of phenolic compounds on ccl 4 induced. Over the last four decades, an extensive body of literature regarding lipid peroxidation has shown its important role in cell biology and human health. Polyenylphosphatidylcholine ppc prevents hepatic lipid peroxidation and attenuates associated injury induced by ccl 4 in rats and by alcohol in baboons, m.
Mechanism for the protective effects of silymarin against carbon tetrachlorideinduced lipid peroxidation and hepatotoxicity in mice. Colchicine prevents changes in the physicochemical properties of liver plasma membranes induced by longterm ccl4 treatment, probably by blocking peroxidation of unsaturated fatty acids. Ilfre was administered orally at 40, 80, and 120 mgkg to rats for 4 weeks. In our experiments, major decrease in lipid peroxidation and consequent reduction in tbars were obtained by treatment with rutin. The liver is not the only target organ of ccl4 toxicity, but there are other organs such as the kidney.
Turmeric extract and its active compound, curcumin. Chronic hepatic stress was induced by a single intraperitoneal injection of ccl4 0. Kolaviron, a biflavonoid fraction of the defatted alcoholic extract of garcinia kola seed, inhibits the. These results indicate that ap has a significant protective effect against acute hepatotoxicity induced by ccl4 in mice, which may be due to its free radical scavenging effect, inhibition of lipid peroxidation, and its ability to increase antioxidant activity. Peroxidation of membrane lipids in the liver was significantly prevented by cll 100, 200, and 300 mgkgday on tissue lipid peroxidation assay and immunostaining with anti4hne antibody. The aim of this study was to investigate whether nacetyl cysteine nac has a protective effect on the toxicity caused by carbon tetrachloride. Lipid peroxidation is a wellestablished mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. The protective effect of nacetyl cysteine against carbon. Changes in the lipid composition of liver plasma membranes were significantly correlated with lipid peroxidation. Hispolon protects against acute liver damage in the rat by inhibiting lipid peroxidation, proinflammatory cytokine, and oxidative stress and downregulating the expressions of inos, cox2, and mmp9.
Effects of aqueous extract of sarcopoterium spinosum on lipid. Pdf the aim of this work was to determine if the action mechanism of gadolinium on ccl4induced liver damage is by preventing lipid peroxidation. Lipid peroxidation can be described generally as a process under which oxidants such as free radicals attack lipids containing carboncarbon double bonds, especially polyunsaturated fatty acids pufas. Pdf the aim of this work was to determine if the action mechanism of gadolinium on ccl4 induced liver damage is by preventing lipid peroxidation. Pdf prevention of ccl4induced liver damage by ginger. The liver was excised and fixed in formalin for histological assessment of hepatic damage. Mda of ccl4treated hamsters was significantly p ccl4 was significantly p ccl4 group table 2. Jci relationship of oxygen and glutathione in protection.
Ten rats were intragastrically administered for 14 days with a grape. Taken together, our results suggest that lipid peroxidation is primarily implicated in ccl 4 induced early mitochondrial injury. The role of ros has been implicated in many human degenerative diseases, including aging and cancer 6,7. Relationship of oxygenand gluta thione in protection against carbon tetrachlorideinduced hepatic microsomal lipid peroxidation andcovalent binding in the rat rationale for the useof hyperbaric oxygen to treat carbontetrachloride ingestion raymondf. Under acidic and mildtemperature conditions, 1methyl2phenylindole was found to react with malondialdehyde mda and 4hydroxyalkenals to yield a stable chromophore with intense maximal absorbance at 586 nm. A number of investigators have utilized this chemical to produce liver cirrhosis in experimental animals parola et al. Data of the present study indicated that lipid peroxidation induced by. Detection of cytochrome p450 gene expression in human placenta in first trimester of. Pdf kupffer cells inhibition prevents lipid peroxidation. Reactions of 1methyl2phenylindole with malondialdehyde.
Further studies are needed to clarify the precise mechanisms of gte action. Prevention of ccl4induced liver damage by ginger, garlic and vitamin e. Hepatoprotective effect of black seed nigella sativa oil. Hepatocytes are reportedly susceptible to the injurious effects of oxidants when exposed to toxic substances such as carbontetrachloride ccl4. Prevention of ccl4induced liver damage by ginger, garlic. The hepatoprotective effects of bele may be due to both the inhibition of lipid peroxidation and the increase of antioxidant activity.
Ccl4 induced a moderate elevation of aminotransferases, swelling of centrilobular hepatocytes, lipid peroxidation, reduction of cytochrome p4502e1 mrna levels and a massive increase in mrna. Treatment with pcee significantly and dose dependently prevented drug induced increase in serum levels of hepatic enzymes. Lipid peroxidation was also determined by estimating the. Administration of ccl4 induced a loss of glutathione from the liver and a decrease in its hepatic content. Several methods have often been used to study free radicalinduced lipid peroxidation following ccl 4 induction suffer. Hepatoprotective effect of ethanol extract from berchemia.
This should result in diminished ccl4induced lipid peroxidation and liver damage. Pdf antioxidant, hepatoprotective and lipid lowering. Silymarin 800 iugml decreased by 70% in vitro lipid peroxidation mediated by ccl4 metabolites, and decreased by 90% peroxidation mediated by nadph alone. Green tea extract attenuates ccl4induced hepatic injury.
Nevertheless it was inhibited by flavonoids and structureactivity relationships were established. Reversal of carbon tetrachloride induced changes in. Carbon tetrachloridemediated lipid peroxidation induces early mitochondrial alterations in mouse liver article pdf available in laboratory investigation 923. Relationship of oxygen and glutathione in protection. Ccl4 treatment in rats reduced the levels of tissue gsh as compared to the rats treated with ccl4 and plant extracts. Calciumdeficient diet attenuates carbon tetrachloride. The use of methanesulfonic acid results in optimal yields of chromophore produced from mda as well as from 4hydroxynonenal. Drinking gte has a beneficial effect decreasing lipid peroxidation, improving gsh, adh, cytochrome p450 reductase activities and downregulating p53 expression in hepatocytes. The widespread claims of the medicinal efficacy of various parts of moringa oleifera plant have been. The aim of this study is to investigate whether a cadeficient diet has an attenuating effect on carbon tetrachloride ccl4induced hepatotoxicity. Carbon tetrachlorideinduced lipid peroxidation and. Carbon tetrachloride ccl 4induced lipid peroxidation and liver injury is a classic experimental model for comprehending the cellular mechanisms behind oxidative injury, and further estimating the therapeutic potential of drugs and antioxidants. Amelioration of ccl4 induced liver injury in swiss albino. Possible protective effect of kolaviron on ccl4induced.
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